2款GMP級DNA聚合酶
2018-08-30文章來(lái)源：Takarabiomed
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■ 制品內容
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TaKaRa Taq Hot Start Version GMP grade
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250 U (5 U/μl)
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10X PCR Buffer (Mg2+ plus) GMP grade
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1 ml
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10X PCR Buffer (Mg2+ free) GMP grade
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1 ml
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25 mM MgCl2 GMP grade
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1 ml
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■ 制品說(shuō)明
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TaKaRa Taq Hot Start Version GMP grade DNA polymerase在嚴格的標準下制造，以確保高質(zhì)量和一致性，符合GMP規定和指南的設施。GMP級別為熱啟動(dòng)聚合酶帶來(lái)了更高水平的可信度和一致性，特別適用于苛刻的研究應用，包括多重PCR。 此GMP級試劑提供：
· 嚴格的規格
· 卓越的性能
· 可定制包裝
所有的Hot-start型Takara Taq聚合酶是抗Taq單克隆抗體和TaKaRa Taq 的混合制品，適用于Hot Start PCR。Hot Start PCR可以提高特異性，降低背景，可在室溫下配制反應液。因Hot Start PCR可防止非特異性擴增，高特異性是實(shí)驗成功的關(guān)鍵，因此是多重PCR的理想選擇?？?em style="margin:0px;padding:0px;list-style-type:none;outline:none;border:none;font-family:"">Taq單克隆抗體在PCR反應最初的DNA變性步驟已變性，因此在常規PCR反應條件下即可使用。
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Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711,6,127,155 and claims outside the US corresponding to expired US Patent No.5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. No right under any other patent claims, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration,is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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■ 保存
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-20℃。避免反復凍融。
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■ 特點(diǎn)
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Test Items
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Specifications
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Results
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Purity (microfluidic chip analysis)
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>98%
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Passed
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Ribonucleases
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No observable degradation
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Passed
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Endonucleases
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No observable degradation
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Passed
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Exonucleases
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No observable degradation
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Passed
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Nicking activities
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No observable relaxation of supercoiled DNA
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Passed
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Residual E. coli gDNA
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<10 copies/1.25 U
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Passed
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Hot-start activity
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Confirmed
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Passed
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PCR performance
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8-kb fragment was amplified
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Passed
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Sensitivity (λ DNA)
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0.5-kb fragment was amplified from 10 pg of λ DNA
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Passed
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Sensitivity (human gDNA)
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0.1-kb fragment was amplified from 30 pg of human
genomic DNA
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Passed
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■ 應用
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· Hot-start PCR
· Multiplex PCR
· qPCR
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■ 制品內容
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50X Titanium Taq SP DNA Polymerase GMP grade
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100 μl
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10X Titanium Taq SP PCR Buffer GMP grade
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600 μl
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■ 制品說(shuō)明
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Titanium® Taq SP DNA Polymerase GMP grade在嚴格的GMP標準下制造，以確保高質(zhì)量和一致性，符合GMP規定和指南的設施。GMP級別為熱啟動(dòng)聚合酶帶來(lái)了更高水平的可信度和一致性，特別適用于苛刻的研究應用，包括多重PCR。 此GMP級試劑提供：
· 嚴格的規格
· 卓越的性能
· 可定制包裝
Titanium® Taq SP DNA Polymerase GMP grade是用于所有PCR應用的高度靈敏，強大的酶。 它適用于任何DNA模板，包括細菌、質(zhì)粒DNA、cDNA以及復雜基因組DNA。 Titanium Taq包括TaqStart抗體，可提高產(chǎn)物的特異性和產(chǎn)量。
Titanium® Taq SP DNA Polymerase GMP grade缺乏野生型Taq的5'外切核酸酶活性，這使得它比其他Taq聚合酶擴增性能更強大，并且可用于擴增高度復雜的DNA混合物。 這種新型酶還包含精心設計的氨基酸替代物，增加其溶解度，使其成為高靈敏度的PCR聚合酶。
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■ 制品特點(diǎn)
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· 更短循環(huán)圈數即可擴增目的片段，同時(shí)降低背景
· 無(wú)需優(yōu)化反應條件-Titanium Taq可在寬廣的Mg離子范圍內進(jìn)行擴增
· 可從高復雜模板中擴增多達2 kb靶基因，如哺乳動(dòng)物基因組DNA
· 當模板為中等豐度的cDNA或不復雜的基因組DNA時(shí)，可以擴增高達4 kb的靶基因
· 可擴增珍貴或低拷貝基因
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■ 保存
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-20℃。避免反復凍融。
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■ 應用
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· Hot-start PCR
· Multiplex PCR
· Genotyping
· Preparative PCR
· Whole-genome PCR
· Primer extension
· Rare template amplification
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產(chǎn)品詳情請點(diǎn)擊以下鏈接：
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http://www.clontech.com/US/Products/PCR/GMP-Grade_Products/Titanium_Taq_SP_GMP_grade
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GMP級別PCR聚合酶產(chǎn)品選擇指南：
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http://www.takarabiomed.com.cn/ProductShow.aspx?m=20161209091818673926&
productID=20180131131045950905
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