人原代肝臟細胞培養基
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人原代肝臟細胞(Human primary hepatocytes, hphep) 是肝臟疾病毒理學(xué)研究和相關(guān)藥物研發(fā)的有力工具。對肝臟疾病模型研究和肝毒性檢測而言,一個(gè)穩健的hphep培養體系至關(guān)重要。然而,當前可用的培養體系培養的肝臟細胞在一周內就喪失了功能性和活性,嚴重限制了原代肝臟細胞的應用。而且,這些培養基維持培養中還需要每天換液。
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Cellartis Power Primary HEP Medium是專(zhuān)門(mén)制定的、用于長(cháng)期維持培養的人原代肝臟細胞培養基。凍存復蘇后接種的hphep,在方便操作的2D培養中,可以存活并維持功能特性4周。此外,這款培養基進(jìn)一步簡(jiǎn)化了肝細胞培養,提供一個(gè)周末免換液的培養日程。
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■ 產(chǎn)品特點(diǎn)
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· 延長(cháng)培養時(shí)間—原代肝臟細胞可以存活并維持功能特性長(cháng)達4周,經(jīng)過(guò)CYP 活性、 albumin分泌
和CYP誘導能力檢測驗證。
· 形式靈活—冷凍運輸,可以被分裝和再次凍存。
· 操作簡(jiǎn)便—周末免換液的培養日程, 使用隔天換液 (周一,周三,周五) 而不是每天換液,
無(wú)需準備復雜的overlays或sandwich培養。
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■ 產(chǎn)品應用
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· Liver disease modeling
· Hepatotoxicity assessment
· Chronic toxicity
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■ 產(chǎn)品信息
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Code No.
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Product
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Size
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Y20020
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Cellartis® Power? Primary HEP Medium
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250 ml
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■ 產(chǎn)品詳情
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http://www.clontech.com/CN/Products/Cell_Biology_and_Epigenetics/Miscellaneous/Cell_Culture/
Primary_Hepatocyte_Medium?sitex=10022:22372:US
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使用Cellartis Power Primary HEP Medium分別培養來(lái)源于三個(gè)不同品牌的、復蘇接種的人原代肝臟細胞。
Cryoplateable human primary hepatocytes from three different vendors (1, 2, and 3) cultured in Cellartis Power Primary HEP Medium. Cells were cultured as described and images were taken 1 (Panel A), 7 (Panel B), 14 (Panel C), 21 (Panel D) and 28 (Panel E) days after thawing. For all images, the scale bar is 100 μm.
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(以上圖片來(lái)源于Takara Bio USA, Inc.)
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